| dc.contributor |
Harvard University--MIT Division of Health Sciences and Technology |
|
| dc.contributor |
Yun, Seok Hyun (Andy) |
|
| dc.creator |
Scarcelli, Giuliano |
|
| dc.creator |
Kim, Pilhan |
|
| dc.creator |
Yun, Seok Hyun (Andy) |
|
| dc.date |
2014-12-10T19:43:23Z |
|
| dc.date |
2014-12-10T19:43:23Z |
|
| dc.date |
2011-09 |
|
| dc.date |
2011-03 |
|
| dc.date.accessioned |
2023-03-01T18:08:16Z |
|
| dc.date.available |
2023-03-01T18:08:16Z |
|
| dc.identifier |
00063495 |
|
| dc.identifier |
1542-0086 |
|
| dc.identifier |
http://hdl.handle.net/1721.1/92262 |
|
| dc.identifier |
Scarcelli, Giuliano, Pilhan Kim, and Seok Hyun Yun. “In Vivo Measurement of Age-Related Stiffening in the Crystalline Lens by Brillouin Optical Microscopy.” Biophysical Journal 101, no. 6 (September 2011): 1539–1545. © 2011 Biophysical Society |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/278890 |
|
| dc.description |
The biophysical and biomechanical properties of the crystalline lens (e.g., viscoelasticity) have long been implicated in accommodation and vision problems, such as presbyopia and cataracts. However, it has been difficult to measure such parameters noninvasively. Here, we used in vivo Brillouin optical microscopy to characterize material acoustic properties at GHz frequency and measure the longitudinal elastic moduli of lenses. We obtained three-dimensional elasticity maps of the lenses in live mice, which showed biomechanical heterogeneity in the cortex and nucleus of the lens with high spatial resolution. An in vivo longitudinal study of mice over a period of 2 months revealed a marked age-related stiffening of the lens nucleus. We found remarkably good correlation (log-log linear) between the Brillouin elastic modulus and the Young's modulus measured by conventional mechanical techniques at low frequencies (∼1 Hz). Our results suggest that Brillouin microscopy is potentially useful for basic and animal research and clinical ophthalmology. |
|
| dc.description |
National Institutes of Health (U.S.) (Grant R21EB008472) |
|
| dc.description |
United States. Dept. of Defense (Grant FA9550-04-1-0079) |
|
| dc.description |
National Science Foundation (U.S.) (Grant CBET-0853773) |
|
| dc.description |
CIMIT: Center for Integration of Medicine and Innovative Technology |
|
| dc.description |
Milton Foundation |
|
| dc.format |
application/pdf |
|
| dc.language |
en_US |
|
| dc.publisher |
Elsevier |
|
| dc.relation |
http://dx.doi.org/10.1016/j.bpj.2011.08.008 |
|
| dc.relation |
Biophysical Journal |
|
| dc.rights |
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use. |
|
| dc.source |
Elsevier |
|
| dc.title |
In Vivo Measurement of Age-Related Stiffening in the Crystalline Lens by Brillouin Optical Microscopy |
|
| dc.type |
Article |
|
| dc.type |
http://purl.org/eprint/type/JournalArticle |
|