| dc.contributor |
Massachusetts Institute of Technology. Department of Mechanical Engineering |
|
| dc.contributor |
Braff, William |
|
| dc.contributor |
Buie, Cullen R. |
|
| dc.creator |
Willner, Dana |
|
| dc.creator |
Hugenholtz, Philip |
|
| dc.creator |
Rabaey, Korneel |
|
| dc.creator |
Buie, Cullen R. |
|
| dc.creator |
Braff, William |
|
| dc.date |
2014-01-06T19:25:00Z |
|
| dc.date |
2014-01-06T19:25:00Z |
|
| dc.date |
2013-10 |
|
| dc.date |
2013-03 |
|
| dc.date.accessioned |
2023-03-01T18:04:27Z |
|
| dc.date.available |
2023-03-01T18:04:27Z |
|
| dc.identifier |
1932-6203 |
|
| dc.identifier |
http://hdl.handle.net/1721.1/83513 |
|
| dc.identifier |
Braff, William A., Dana Willner, Philip Hugenholtz, Korneel Rabaey, and Cullen R. Buie. “Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties.” Edited by Arum Han. PLoS ONE 8, no. 10 (October 16, 2013): e76751. |
|
| dc.identifier |
https://orcid.org/0000-0002-2275-4570 |
|
| dc.identifier |
https://orcid.org/0000-0001-9529-2912 |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/278643 |
|
| dc.description |
Insulator-based dielectrophoresis can be used to manipulate biological particles, but has thus far found limited practical applications due to low sensitivity. We present linear sweep three-dimensional insulator-based dielectrophoresis as a considerably more sensitive approach for strain-level discrimination bacteria. In this work, linear sweep three-dimensional insulator-based dielectrophoresis was performed on Pseudomonas aeruginosa PA14 along with six isogenic mutants as well as Streptococcus mitis SF100 and PS344. Strain-level discrimination was achieved between these clinically important pathogens with applied electric fields below 10 V/mm. This low voltage, high sensitivity technique has potential applications in clinical diagnostics as well as microbial physiology research. |
|
| dc.description |
National Science Foundation (U.S.) (Award 1150615) |
|
| dc.description |
MISTI Hayashi Seed Fund |
|
| dc.format |
application/pdf |
|
| dc.language |
en_US |
|
| dc.publisher |
Public Library of Science |
|
| dc.relation |
http://dx.doi.org/10.1371/journal.pone.0076751 |
|
| dc.relation |
PLoS ONE |
|
| dc.rights |
http://creativecommons.org/licenses/by/2.5/ |
|
| dc.source |
PLoS |
|
| dc.title |
Dielectrophoresis-Based Discrimination of Bacteria at the Strain Level Based on Their Surface Properties |
|
| dc.type |
Article |
|
| dc.type |
http://purl.org/eprint/type/JournalArticle |
|