| dc.contributor |
EPSRC - Engineering and Physical Sciences Research Council |
|
| dc.contributor |
The Royal Society |
|
| dc.contributor |
Clarke, Dave |
|
| dc.creator |
Gallagher, Kelly |
|
| dc.creator |
Palasser, Michael |
|
| dc.creator |
Clarke, David |
|
| dc.date |
2018-09-27T14:56:24Z |
|
| dc.date |
2018-09-27T14:56:24Z |
|
| dc.date.accessioned |
2023-02-17T20:51:14Z |
|
| dc.date.available |
2023-02-17T20:51:14Z |
|
| dc.identifier |
Gallagher, Kelly; Palasser, Michael; Clarke, David. (2018). Isotope Depletion Mass Spectrometry (ID MS) for Enhanced Top-Down Protein Fragmentation - Datasets, [dataset]. University of Edinburgh. School of Chemistry. https://doi.org/10.7488/ds/2446. |
|
| dc.identifier |
https://hdl.handle.net/10283/3190 |
|
| dc.identifier |
https://doi.org/10.7488/ds/2446 |
|
| dc.identifier.uri |
http://localhost:8080/xmlui/handle/CUHPOERS/243857 |
|
| dc.description |
Top-down mass spectrometry has become an important technique for the identification of proteins and characterisation of chemical and posttranslational modifications. However, as the molecular mass of proteins increases intact mass determination and top-down fragmentation efficiency become more challenging due to the partitioning of the mass spectral signal into many isotopic peaks. In large proteins, this results in reduced sensitivity and increased spectral complexity and signal overlap. This phenomenon is a consequence of the natural isotopic heterogeneity of the elements which comprise proteins (notably 13C). Here we present a bacterial recombinant expression system for the production of proteins depleted in 13C and 15N and use this strategy to prepare a range of isotopically depleted proteins. High resolution MS of isotope depleted proteins reveal dramatically reduced isotope distributions, which results in increases in sensitivity and deceased spectral complexity. We demonstrate that the monoisotopic signal is observed in mass spectra of proteins up to ~50 kDa. This allows confidant assignment of accurate molecular mass, and facile detection of low mass modifications (such as deamidation).
We outline the benefits of this isotope depletion strategy for top-down fragmentation. The reduced spectral complexity alleviates problems of signal overlap; the presence of monoisotopic signals allow more accurate assignment of fragment ions; and the dramatic increase in single-to-noise ratio (up to 7-fold increases) permits vastly reduced data acquisition times. Together, these compounding benefits allow the assignment of ca. 3-fold more fragment ions than analysis of proteins with natural isotopic abundances. Thus, more comprehensive sequence coverage can be achieved; we demonstrate near single amino-acid resolution of the 29 kDa protein carbonic anhydrase from a single top-down MS experiment. Finally, we demonstrate that the ID-MS strategy allows far greater sequence coverage to be obtained in time limited top-down data acquisitions – highlighting potential advantages for top-down LC-MS/MS workflows and top-down proteomics. |
|
| dc.description |
Please refer to Readme file for full details. All data files are .baf format and can be opened with DataAnalysis (Bruker Daltonics). |
|
| dc.format |
application/pdf |
|
| dc.format |
application/zip |
|
| dc.language |
eng |
|
| dc.publisher |
University of Edinburgh. School of Chemistry |
|
| dc.rights |
Creative Commons Attribution 4.0 International Public License |
|
| dc.source |
All files all included as .baf files (Bruker Daltonics). May be viewed using DataAnalysis (Bruker Daltonics) |
|
| dc.subject |
Mass Spectrometry |
|
| dc.subject |
Top-Down Mass Spectrometry |
|
| dc.subject |
Isotope Depletion |
|
| dc.subject |
Electron Capture Dissociation |
|
| dc.subject |
Protein Structure |
|
| dc.subject |
FT-ICR MS |
|
| dc.subject |
Physical Sciences::Analytical Chemistry |
|
| dc.title |
Isotope Depletion Mass Spectrometry (ID MS) for Enhanced Top-Down Protein Fragmentation - Datasets |
|
| dc.type |
dataset |
|